Source: Bovine (Swine) pancreas
General description
Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).
Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.
The high purity Chymotrypsin is purified by re-crystallization, and then by Ion Exchange Chromatography and ultra-filtration.
Specification
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Items
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Specification
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Method
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Appearance
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White or almose white lyophilized powder
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Solvent Transparentness
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Confroms
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USP30
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Loss on drying
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≤ 5.0%
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USP30
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Residue on Ignition
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≤2.5%
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USP30
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Microbial limits
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Confroms
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USP30
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Trypsin
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≤5%
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USP30
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Assay
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Chymotrypsin≥1000 USPunits/mg powder
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USP30
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Storage
Sealed, Dark, at temperature 2-8℃
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