Deoxyribonuclease
Source: Bovine Pancrease
General description
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+
Specification
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Items
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Specification
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Method
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Appearance
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White or pale yellow lyophilized powder
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Loss on drying
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≤ 0.3%
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K.P
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Residue on Ignition
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≤1.0%
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K.P
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Assay
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No less than 500Kunitz units/mg powder
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Sigma-Aldrich
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Storage：Sealed, Dark, at temperature 2-8℃
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